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Human NBL1 / DAND1 / DAN Protein (Fc Tag)

D1S1733E,DAN,DAND1,NB,NO3

Catalog Number P10169-H02H
Organism Species Human
Host Human Cells
Synonyms D1S1733E,DAN,DAND1,NB,NO3
Molecular Weight The recombinant human DAN/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and has a calculated molecular mass of 44.4 kDa. As a result of glycosylation, rh DAN/Fc monomer migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.
predicted N Ala 16
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human DAN precursor (NP_005371.1) (Met 1-Asp 180) was expressed with C-terminal fused Fc region of human IgG1.
Bio-activity Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.2-1.2 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.
Research Area Cancer |Oncoprotein & suppressor & biomarker |Tumor biomarker |Tumor Suppressor |Other in Tumor suppressor
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante and PRDC (protein related to DAN and cereberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition, DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.
Reference
  • Ozaki T, et al. (1995) Overexpression of DAN gene product in normal rat fibroblasts causes a retardation of the entry into the S phase. Cancer Res. 55(4): 895-900.
  • Nakamura Y, et al. (1997) A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. Eur J Cancer. 33(12): 1986-90.
  • Ogita J, et al. (2001) Expression of the Dan gene during chicken embryonic development. Mech Dev. 109(2): 363-5.
  • Kim AS, et al. (2003) Expression of the BMP antagonist Dan during murine forebrain development. Brain Res Dev Brain Res. 145(1): 159-62.