Human NKG2D / CD314 Protein (aa 78-216, His Tag)

CD314,D12S2489E,KLR,NKG2-D,NKG2D

100ug (NPP2369) Please inquiry

Catalog Number	P10575-H07B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	CD314,D12S2489E,KLR,NKG2-D,NKG2D
Molecular Weight	The recombinant human NKG2D consists of 157 amino acids and has a calculated molecular mass of 18.4 kDa.
predicted N	His
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human NKG2D (Phe78-Val216) was fused with a polyhistide tag at the N-terminus.
Bio-activity	1. Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch (P10679-H03H), The EC₅₀ of human ULBP1-Fch (P10679-H03H) is 0.04-0.08 μg/ml. 2. Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch (P10759-H03H), The EC₅₀ of human MICB-Fch (P10759-H03H) is 15.9-37.1 ng/ml.
Research Area	Cancer \|Signal transduction \|Signaling Pathway \|G Protein Signaling \|Small G Protein \|Ras GTPase Family \|
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.
Reference	Houchins J, et al. (1991) DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J Exp Med. 173: 1017-102. Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428):727-9. Zafirova B, et al. (2011) Regulation of immune cell function and differentiation by the NKG2D receptor. Cell Mol Life Sci. 68(21):3519-29.