Human NUDT5 / ADP-sugar Pyrophosphatase Protein (His Tag)

hYSAH1,YSA1,YSA1H,YSAH1

Catalog Number	P13068-H07E
Organism Species	Human
Host	E. coli
Synonyms	hYSAH1,YSA1,YSA1H,YSAH1
Molecular Weight	The recombinant human NUDT5 consists of 234 amino acids and predicts a molecular mass of 26.3 KDa. It migrates as an approximately 35 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human NUDT5 (Q9UKK9) (Glu2-Phe219) was expressed with a polyhistidine tag at the N-terminus.
Bio-activity
Research Area	Epigenetics \|DNA / RNA \|DNA / Nucleotides
Formulation	Lyophilized from sterile 50mM Tris, 10% glycerol, pH 8.0. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	ADP-sugar Pyrophosphatase, also known as NUDT5, eliminates toxic nucleotide derivatives from the cell and regulate the levels of important signaling nucleotides and their metabolites. NUDT5 functions as a MutT-related protein and catalyzes the hydrolysis of 8-oxoGDP to 8-oxoGMP, thereby preventing misincorporation of 8-oxoGua into RNA. NUDT5 may play significant roles in regulating the G1-S transition in mammalian cells. It can also hydrolyze other nucleotide sugars with low activity.
Reference	Ishibashi T. et al., 2004, EMBO Rep. 4 (5): 479-8. Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7. Rush J. et al., 2005, Nat Biotechnol. 23 (1): 94-101. Kamiya H. et al., 2009, DNA Repair. 8 (10): 1250-4.