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Human Neuropilin-1 / NRP1 Protein (Fc Tag)

BDCA4,CD304,Neuropilin-1,NP1,NRP,VEGF165R

Catalog Number P10011-H02H
Organism Species Human
Host Human Cells
Synonyms BDCA4,CD304,Neuropilin-1,NP1,NRP,VEGF165R
Molecular Weight The recombinant human NRP1/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 861 amino acids and has a calculated molecular mass of 96.5 kDa. As a result of glycosylation, rh NRP1/Fc monomer migrates as an approximately 120-125 kDa protein in SDS-PAGE under reducing conditions.
predicted N Phe 22
SDS-PAGE
Purity > 85 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human NRP1 isoform b (NP_001019799.1) (Met 1-Lys 644) was expressed with the Fc region of human IgG1 at the C-terminus.
Bio-activity Measured by its ability to bind with recombinant human VEGF165 in a functional ELISA.
Research Area Cancer |Oncoprotein & suppressor & biomarker |Oncoprotein |Growth Factor & Receptor |Vascular Endothelial Growth Factor (VEGF) & Receptor |VEGF Receptor |
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Neuropilin is a type I transmembrane protein and the molecular mass is 120 kDa. Two homologues, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.
Reference
  • Nakamura F, et al. (2002) Structural and functional relation of neuropilins. Adv Exp Med Biol. 515: 55-69.
  • Romeo PH, et al. (2002) Neuropilin-1 in the immune system. Adv Exp Med Biol. 515: 49-54.
  • Klagsbrun M, et al. (2002) The role of neuropilin in vascular and tumor biology. Adv Exp Med Biol. 515: 33-48.
  • Staton CA, et al. (2007) Neuropilins in physiological and pathological angiogenesis. J Pathol. 212(3): 237-48.
  • Bagri A, et al. (2009) Neuropilins in tumor biology. Clin Cancer Res. 15(6): 1860-4.