Human Noggin / NOG Protein (His Tag)
Noggin,SYM1,SYNS1
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| Catalog Number | P10267-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | Noggin,SYM1,SYNS1 |
| Molecular Weight | The recombinant human Noggin comprises 216 amino acids after removal of the signal peptide and has a predicted molecular mass of 24.6 kDa. As a result of glycosylation, rhNoggin migrates as an approximately 30 kDa bnd in SDS-PAGE under reducing conditions. |
| predicted N | Gln 28 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its ability to inhibit recombinant human BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.05-0.3 μg/mL in the presence of 50 ng/mL of recombinant human BMP4. |
| Research Area | Immunology |Cytokines & Growth Factors |Cytokine & Receptor |Transforming Growth Factor Beta (TGF-beta) Superfamily |TGF-beta Superfamily Modulators |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities, with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs, Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7, and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes, including embryonic dorsal-ventral axis, induction of neural tissue, formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion. |
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