Call Now

Human PDE1B Protein (His & GST Tag)

PDE1B1,PDES1B

Catalog Number P11746-H20B
Organism Species Human
Host Baculovirus-Insect Cells
Synonyms PDE1B1,PDES1B
Molecular Weight The recombinant human PDE1B/GST chimera consists of 773 amino acids and has a calculated molecular mass of 89.2 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
predicted N Met
SDS-PAGE
Purity > 94 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human PDE1B long isoform (Q01064-1) (Met 1-Asp 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity
Research Area Immunology |Signal Transduction |Second Messenger |Nucleotide Messenger |cGMP
Formulation Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, also known as Cam-PDE 1B and PDE1B, is a cytoplasm protein which belongs to the cyclic nucleotide phosphodiesterase family and PDE1 subfamily. Phosphodiesterase-10A (PDE10A), Phosphodiesterase-1B (PDE1B), Phosphodiesterase-4B (PDE4B), and Phosphodiesterase-4A (PDE4A) are important regulators of signal transduction in striatum due to their catalysis of cyclic AMP and cyclic GMP. PDE1B is highly expressed in the striatum. It binds two divalent metal cations per subunit. Site one of PDE1B may preferentially bind zinc ions, while site two of PDE1B has a preference for magnesium and/or manganese ions. PDE1B is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. It has a preference for cGMP as a substrate.
Reference
  • Reed,T.M. et al.,1998, Mamm Genome. 9 (7):571-6.
  • Zhang K.Y.J. et al., 2004, Mol. Cell 15:279-86.
  • Siuciak,J.A. et al., 2007, Neuropharmacology. 53 (1):113-24.
  • Dlaboga, D. et al., 2008, Neuropharmacology. 54 (4):745-54.