Human PDGFRB / CD140b Protein (His & Fc Tag)

CD140B,IBGC4,IMF1,JTK12,KOGS,PDGFR,PDGFR-1,PDGFR1,PENTT

• 100ug (NPP4159) Please inquiry

Catalog Number	P10514-H03H
Organism Species	Human
Host	Human Cells
Synonyms	CD140B,IBGC4,IMF1,JTK12,KOGS,PDGFR,PDGFR-1,PDGFR1,PENTT
Molecular Weight	The secreted recombinant human PDGFRβ/Fc is a disulfide-linked homodimer. The reduced monomer comprises 747 amino acids and predicts a molecular mass of 84.2 kDa. It migrates as an approximately 125-135 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Leu 33
SDS-PAGE
Purity	> 70 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human PDGFRβ (NP_002600.1) extracellular domain (Met 1-Lys 531) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Bio-activity
Research Area	Cancer |Invasion microenvironment |Angiogenesis |Growth Factor & Receptor |Platelet-Derived Growth Factor (PDGF) & Receptor |PDGF Receptor |
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.
Reference	Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5. Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. Suzuki S, et al. (2010) Clinicopathological significance of platelet-derived growth factor (PDGF)-B and vascular endothelial growth factor-A expression, PDGF receptor- phosphorylation, and microvessel density in gastric cancer. BMC cancer. 10: 659.