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Human PDHA1 / C54G Protein1 (aa 30-390)

PDHA,PDHAD,PDHCE1A,PHE1A

Catalog Number P14567-HNCB
Organism Species Human
Host Baculovirus-Insect Cells
Synonyms PDHA,PDHAD,PDHCE1A,PHE1A
Molecular Weight The recombinant human PDHA1 consists of 363 amino acids and has a calculated molecular mass of 40.4 kDa. The recombinant protein migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
predicted N Gly
SDS-PAGE
Purity > 85 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human PDHA1 (P08559-1) (Phe30-Ser390) was fused with two additional amino acids (Gly & Pro) at the N-terminus.
Bio-activity
Research Area Epigenetics |Cell cycle |Markers
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background PDHA1, also known as C54G1, is an alpha subunit of pyruvate dehydrogenase. Pyruvate dehydrogenase, together with dihydrolipoamide acetyltransferase and lipoamide dehydrogenase, composes the pyruvate dehydrogenase (PDH) complex. The PDH complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. PDHA1 plays a key role in the function of the PDH complex. Defects in PDHA1 can cause pyruvate dehydrogenase E1-alpha deficiency. Defects in PDHA1 also are the cause of X-linked Leigh syndrome (X-LS). X-LS is an early-onset progressive neurodegenerative disorder with a characteristic neuropathology consisting of focal, bilateral lesions in one or more areas of the central nervous system, including the brainstem, thalamus, basal ganglia, cerebellum, and spinal cord.
Reference
  • Quintana E. et al., 2010, Clin Genet. 77 (5): 474-82.
  • Ah Mew N. et al., 2011, Pediatr Neurol. 45 (1): 57-9.
  • Pinheiro A. et al., 2012, Gene. 506 (1): 173-8.