Human PFDN4 / PFD4 Protein (His Tag)

C1,PFD4

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Catalog Number	P14144-H07E
Organism Species	Human
Host	E. coli
Synonyms	C1,PFD4
Molecular Weight	The recombinant human PFDN4 consists of 149 amino acids and predicts a molecular mass of 17.2 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 80 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human PFDN4 (Q9NQP4 ) (Met1-Ser134) was expressed with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Signaling |Signal Transduction |Protein Trafficking |Chaperones |Other Chaperones
Formulation	Lyophilized from sterile PBS, 5% Glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	PFDN4 is a member of the prefoldin beta subunit family. It is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. PFDN4 binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. PFDN4 also binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Reference	Iijima M, et al. (1996) Cloning of cDNA with possible transcription factor activity at the G1-S phase transition in human fibroblast cell lines. Acta Med Okayama. 50(2):73-7. Hartl FU, et al. (2002) Molecular chaperones in the cytosol: from nascent chain to folded protein. Science. 295(5561):1852-8. Vainberg I, et al. (1998) Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin. Cell. 93(5):863-73.