Human PGA4 / Pepsinogen A Protein (Fc Tag)
FLJ58952,FLJ77962,PGA3,PGA4,PGA5
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| Catalog Number | P13082-H02H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | FLJ58952,FLJ77962,PGA3,PGA4,PGA5 |
| Molecular Weight | The secreted recombinant human PGA4/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 614 amino acids and has a predicted molecular mass of 67.3 kDa. The apparent molecular mass of rhPGA/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Ile 16 |
| SDS-PAGE |  |
| Purity | > 80 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human PGA4 (P00790) (Met 1-Ala 388) was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, AnaSpec, Catalog # 27114. The specific activity is > 3000 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH) |
| Research Area | Cancer |Invasion microenvironment |Adhesion molecule |Extracelluar matrix |others in ECM |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | PGA4 (Pepsinogen 4, group I), or Pepsinogen A, is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed, and does not digest the body's own proteins in the stomach's lining. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin, and prochymosin. There are two major groups of pepsinogen, namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin), and each frequently has isozymogens. The PGA3, PGA4 and PGA5 genes encode identical human pepsinogen A enzymes. |
| Reference |