Human PHOSPHO1 Protein (His Tag)

PHOSPHO1

Catalog Number	P13187-H07E
Organism Species	Human
Host	E. coli
Synonyms	PHOSPHO1
Molecular Weight	The recombinant human PHOSPHO1 consisting of 282 amino acids and has a calculated molecular mass of 31.5 kDa. It migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met 1
SDS-PAGE
Purity	> 92 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human PHOSPHO1 isoform 1 (Q8TCT1-1) (Met 1-Cys 267) was expressed, with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Cardiovascular \|Angiogenesis \|Adhesion Molecules in Angiogenesis \|Extracellular Matrix \|Structures \|Bone \|
Formulation	Lyophilized from sterile PBS, 10% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Stewart AJ, et al. (2003) Comparative modelling of human PHOSPHO1 reveals a new group of phosphatases within the haloacid dehalogenase superfamily. Protein Eng. 16(12): 889-95. Camps M, et al. (2000) Dual specificity phosphatases: a gene family for control of MAP kinase function. FASEB J. 14(1): 6-16. Barford D. (1996) Molecular mechanisms of the protein serine/threonine phosphatases. Trends Biochem Sci. 21(11): 407-12.