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Human PON3 / Paraoxonase 3 Protein (50 Ser/Asn, His Tag)

PON3

Catalog Number P13063-H07B
Organism Species Human
Host Baculovirus-Insect Cells
Synonyms PON3
Molecular Weight The recombinant human PON3 consists of 373 amino acids and predicts a molecular mass of 42 kDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
predicted N Met
SDS-PAGE
Purity > 85 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human PON3 (Q15166-1) (Met 1-Leu 354) (50 Ser/Asn) was expressed, with a polyhistidine tag at the N-terminus.
Bio-activity
Research Area Signaling |Signal Transduction |Metabolism |Pathways and Processes |Redox metabolism |Oxidative stress |
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 1mM CaCL2, 10% gly, 0.1% DDM
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference
  • Draganov DI, et al. (2000) Rabbit serum paraoxonase 3 (PON3) is a high density lipoprotein-associated lactonase and protects low density lipoprotein against oxidation. J Biol Chem. 275(43): 33435-42.
  • Shih DM, et al. (2007) Decreased obesity and atherosclerosis in human paraoxonase 3 transgenic mice. Circ Res. 100(8): 1200-7.
  • Rosenblat M, et al. (2003) Mouse macrophage paraoxonase 2 activity is increased whereas cellular paraoxonase 3 activity is decreased under oxidative stress. Arterioscler Thromb Vasc Biol. 23(3): 468-74.