Human PPARG / Nr1c3 / PPARgamma Protein (His & GST Tag)

CIMT1,GLM1,NR1C3,PPARG1,PPARG2,PPARgamma

Catalog Number	P12019-H20B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	CIMT1,GLM1,NR1C3,PPARG1,PPARG2,PPARgamma
Molecular Weight	The recombinant human PPARG/GST chimera consists of 742 amino acids and has a calculated molecular mass of 85.4 kDa. It migrates as an approximately 85.4 kDa band in SDS-PAGE under reducing conditions as predicted.
predicted N	Met
SDS-PAGE
Purity	> 65 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human PPARG isoform 2 (P37231-1) (Met 1-Tyr 505) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity
Research Area	Cancer \|Signal transduction \|Metabolism \|Types of disease \|Metabolism in Heart disease
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4, 20% gly, 0.3mM DTT 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Hamblin M, et al. (2009) PPARs and the Cardiovascular System. Antioxid Redox Signal. 11 (6): 1415-52. Khateeb J, et al. (2010) Paraoxonase 1 (PON1) expression in hepatocytes is upregulated by pomegranate polyphenols: a role for PPAR-gamma pathway. Atherosclerosis. 208 (1): 119-25. Fajas L, et al. (1997) The organization, promoter analysis, and expression of the human PPARgamma gene. J Biol Chem. 272 (30): 18779-89.