Human PPM1A / PP2CA / PP2C-alpha Protein (His Tag)
PP2C-ALPHA,PP2CA,PP2Calpha
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| Catalog Number | P12984-H08E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | PP2C-ALPHA,PP2CA,PP2Calpha |
| Molecular Weight | The recombinant human PPM1A consisting of 392 amino acids and has a calculated molecular mass of 43.8 kDa. It migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met 1 |
| SDS-PAGE |  |
| Purity | > 85 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human PPM1A isoform alpha-1 (P35813-1) (Met 1-Trp 382) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | Cardiovascular |Heart |Apoptosis |Intracellular |NF-kB (NFkB) Pathway |
| Formulation | Lyophilized from sterile PBS, 10% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis. |
| Reference |