Human PRDM2 / RIZ1 Protein
HUMHOXY1,KMT8,MTB-ZF,RIZ,RIZ1,RIZ2
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| Catalog Number | P11216-HNCE |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | HUMHOXY1,KMT8,MTB-ZF,RIZ,RIZ1,RIZ2 |
| Molecular Weight | The recombinant human PRDM2 consists of 202 amino acids and predicts a molecular mass of 23 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Gly |
| SDS-PAGE |  |
| Purity | > 96 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the N-terminal segment of human PRDM2 (NP_036363.2) (Met 1-Ala 200), containing the SET domian, was expressed, with two additonal amino acids (Gly & Pro) at the N-terminus. |
| Bio-activity | |
| Research Area | Signaling |Signal Transduction |Signaling Pathway |Nuclear signaling |Nuclear Hormone Receptor |Estrogen | |
| Formulation | Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.5mM DTT, 0.5mM GSH, pH 8.0 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | PR domain containing 2, with ZNF domain (PRDM2), also known as zinc finger protein RIZ, is a member of histone methyltransferase (HMT) class enzymes that methylate lysine residues of histones or proteins. HMTs contain a conserved catalytic core termed the SET domain, which shares sequence homology with an independently described sequence motif, the PR domain. PRDM2 contains 8 C2H2-type zinc fingers and a distinct SET domain, and is highly expressed in retinoblastoma cell lines and in brain tumors, as well as in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. PRDM2 is a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3, and is identified as a tumor suppressor. It is reported that intact PR( SET) sequence is required for tumor suppression functions, mutations in the PR domain caused activity reduction in human cancers. Also, S-adenosylhomocysteine or methyl donor deficiency inhibits RIZ1 and other H3 lysine 9 methylation activities. PRDM2 may also function as a DNA-binding transcription factor. It Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and act as a transcriptional activator. In addition, PRDM2 (RIZ) is able to binds to the retinoblastoma protein (RB) and also Interacts with GATA3. |
| Reference |