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Human PTPMT1 Protein (His Tag)

DUSP23,FLJ46081,MOSP,PLIP,PNAS-129

Catalog Number P12902-H07E
Organism Species Human
Host E. coli
Synonyms DUSP23,FLJ46081,MOSP,PLIP,PNAS-129
Molecular Weight The recombinant human PTPMT1 consisting of 190 amino acids and has a calculated molecular mass of 21.7 kDa. It migrates as an 20 kDa band in SDS-PAGE under reducing conditions as predicted.
predicted N Met
SDS-PAGE
Purity > 94 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human PTPMT1 isoform 1 (Q8WUK0-1) (Lys 28-Thr 201) was expressed, with a polyhistidine tag at the N-terminus.
Bio-activity Measured by its ability to cleave pNPP.
The specific activity is >200 pmoles/min/μg.
Research Area Cancer |Signal transduction |Protein Phosphorylation |Tyrosine Phosphatase
Formulation Lyophilized from sterile PBS, 10% glycerol, 1mM DTT, pH 7.5
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background PTPMT1 (PTP localized to the Mitochondrion 1) is a member of the protein tyrosine phosphatase superfamily that is localized exclusively to the mitochondrion. It has been recently reported that PTPMT1 dephosphorylates phosphatidylglycerol phosphate, an essential intermediate of cardiolipin biosynthesis. PTPMT1 deficiency in mouse embryonic fibroblasts compromises mitochondrial respiration and results in abnormal mitochondrial morphology. Lipid analysis of PTPMT1-deficient fibroblasts reveals an accumulation of PGP along with a concomitant decrease in phosphatidylglycerol. Modulation of mitochondrial ATP synthesis by PTPMT1 suggests a novel approach for the treatment of pancreatic cancers, which represent some of the deadliest forms of human tumors. The gluttony of cancer cells for energy is well established, and with the development of a modulator of expression, one may hope that we could also achieve the synthetic induction of PTPMT1 expression. It would then be expected that this effect would attenuate, if not abolish, the growth of pancreas-derived tumor cells and support the establishment of a novel regimen for pancreatic cancers. 
Reference
  • Zhang J, et al. (2011) Mitochondrial phosphatase PTPMT1 is essential for cardiolipin biosynthesis. Cell Metab. 13 (6): 690-700.
  • Boisclair Y, et al. (2005) Firing up mitochondrial activities with PTPMT1. Mol Cell. 19 (3): 291-2.
  • Xiao J, et al. (2011) Structural and functional analysis of PTPMT1, a phosphatase required for cardiolipin synthesis. Proc Natl Acad Sci. 108 (29): 11860-5.