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Human PTPN12 Protein

PTP-PEST,PTPG1

Catalog Number P11556-HNCB
Organism Species Human
Host Baculovirus-Insect Cells
Synonyms PTP-PEST,PTPG1
Molecular Weight The secreted recombinant human PTPN12 consists of 357 amino acids and predicts a molecular mass of 41.8 KDa. The apparent molecular mass of the protein is approximately 41 KDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N Gly
SDS-PAGE
Purity > 85 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
Bio-activity Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site (Catalog # ES006).
The specific activity is >20 µmol/min/mg.
Research Area Signaling |Signal Transduction |Other Related Intracellular Topics |Mitochondrial Proteins
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein–protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobilit.
Reference
  • Garton AJ. et al., 1997, Oncogene. 15 (8): 877-85.
  • Lin Yi. et al., 2003, Am J Physiol Heart Circ. 285 (2): H710-21.
  • Takekawa M. et al., 1994, FEBS Lett. 339 (3): 222-8.