Human PTPRA / PTPalpha Protein (aa 174-793, His & GST Tag)

HEPTP,HLPR,HPTPA,HPTPalpha,LRP,PTPA,PTPRL2,R-PTP-alpha,RPTPA

• 100ug (NPP2445) Please inquiry

Catalog Number	P13175-H20B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	HEPTP,HLPR,HPTPA,HPTPalpha,LRP,PTPA,PTPRL2,R-PTP-alpha,RPTPA
Molecular Weight	The recombinant human PTPRA (aa 174-793)/GST chimera consists of 857 amino acids and has a calculated molecular mass of 99 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human PTPRA isoform 2 (P18433-2) cytoplasmic domain (Ala 174-Lys 793) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity	1. Measured by its ability to bind biotinylated recombinant mouse SRC in a functional ELISA. 2. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >40000 pmol/min/μg.
Research Area	Signaling |Signal Transduction |Phosphatase & Regulator |Phosphatase Regulator
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 3mM DTT 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Kaplan R, et al. (1990) Cloning of three human tyrosine phosphatases reveals a multigene family of receptor-linked protein-tyrosine-phosphatases expressed in brain. Proc Natl Acad Sci U S A. 87 (18): 7000-4. Hertog JD, et al. (1996) Tight association of GRB2 with receptor protein-tyrosine phosphatase alpha is mediated by the SH2 and C-terminal SH3 domains. EMBO J. 15 (12): 3016-27. Ye H, et al. (2011) Receptor-like protein-tyrosine phosphatase a enhances cell surface expression of neural adhesion molecule NB-3. J Biol Chem. 286 (29): 26071-80.