Human Pepsinogen C / PGC Protein (His Tag)
PEPC,PGII
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| Catalog Number | P12072-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | PEPC,PGII |
| Molecular Weight | The secreted recombinant human PGC (pro form) consists of 383 amino acids and has a predicted molecular mass of 42 kDa as estimated in SDS-PAGE under reducing conditions. |
| predicted N | Ala 17 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human PGC (NP_002621.1) (Met 1-Ala 388) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | Cancer |Signal transduction |Metabolism |Types of disease |Metabolism in Cancer |
| Formulation | Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. At low PH conditions, Pepsinogen C undergoes conversion into active enzyme. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. |
| Reference |