Human Periostin / POSTN Protein (His Tag)
OSF-2,OSF2,PDLPOSTN,periostin,PN
- 100ug (NPP4164) Please inquiry
| Catalog Number | P10299-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | OSF-2,OSF2,PDLPOSTN,periostin,PN |
| Molecular Weight | The recombinant human POSTN consists of 771 amino acids and has a predicted molecular mass of 86 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 80 kDa due to glycosylation. |
| predicted N | Asn 22 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human POSTN isoform 3 (NP_001129407.1) corresponding to amino acid (Met 1-Gln 781) was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to POSTN-His coated plates (10μg/mL, 100μL/well), approximately >60% will adhere specifically after 30 minutes at 37℃. |
| Research Area | Signaling |Signal Transduction |Cytoskeleton / ECM |Extracellular Matrix |Structures |Bone | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Periostin ( POSTN ), also known as OSF2 (osteoblast specific factor 2), is a heterofunctional secreted extracellular matrix (ECM) protein comprised of four fasciclin domains that promotes cellular adhesion and movement, as well as collagen fibrillogenesis. Postn is expressed in unique growth centers during embryonic development where it facilitates epithelial-mesenchymal transition (EMT) of select cell populations undergoing reorganization. In the adult, Postn expression is specifically induced in areas of tissue injury or areas with ongoing cellular re-organization. In the adult heart Postn is induced in the ventricles following myocardial infarction, pressure overload stimulation, or generalized cardiomyopathy. Although the detailed function of Postn is still unclear, Postn-integrin interaction is thought to be involved in tumor development. Postn is frequently overexpressed in various types of human cancers, stimulating metastatic growth by promoting cancer cell survival, invasion and angiogenesis, and can be a useful marker to predict the behavior of cancer. |
| Reference |