Human RAC2 Protein (His Tag)
EN-7,Gx,HSPC022,p21-Rac2
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| Catalog Number | P12100-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | EN-7,Gx,HSPC022,p21-Rac2 |
| Molecular Weight | The recombinant human RAC2 comprises 200 amino acids and has a predicted molecular mass of 22.6 kDa. It migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 94 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human RAC2 (P15153) (Met 1-Cys 189) was expressed, with a polyhistidine tag at the N-terminus. |
| Bio-activity | |
| Research Area | Signaling |Signal Transduction |G protein signaling |Small G Protein |Other in Small G Protein |
| Formulation | Lyophilized from sterile PBS, 20% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Ras-related C3 botulinum toxin substrate 2 (Rac2) is a small G-protein belonging to the Ras subfamily of the GTPase family. Rac2 acts as an "on / off" switch for signal transduction cascades and motilities. When GDP is attached to the small G-protein, the enzyme is inactivated. Release of the GDP and replace of the GTP cativate the GTPasee. Rac2 remains active until the GTP is hydrolyzed to GDP. Rac2 is a hematopoietic-specific Rho family GTPase implicated as an important constituent of the NADPH oxidase complex and shares 92% amino acid identity with the ubiquitously expressed Rac1. The small G-protein Rac2 regulates the rearrangements of actin and membrane necessary for Fcy receptor-mediated phagocytosis by macrophages. Activated Rac2 binds to the p21-binding domain of PAK1 and this binding provided a basis for microscopic methods to localize activation of these G proteins inside cells. |
| Reference |