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Human RGMA Protein (His Tag)

RGM

Catalog Number P12086-H08H
Organism Species Human
Host Human Cells
Synonyms RGM
Molecular Weight The secreted recombinant human RGMA consists of 386 amino acids and has a predicted molecular mass of 43 kDa. As a result of glycosylation and proteolytic processing, rhRGMA migrates mainly as an approximately 36 kDa in SDS-PAGE corresponding to the mature segment under reducing conditions, and the un-cleaved pro form can also be seen.
predicted N Cys 48
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human RGMA isoform 3 (NP_064596.2) (Met 1-Gly 422), without the C-terminal pro peptide, was fused with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area Neuroscience |Neurology process |Growth and Development |Axon Guidance |Other Axon Guidance Proteins
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background RGMa, also known as RGM domain family, member A, belongs to the RGM (repulsive guidance molecule) family whose members are membrane-associated glycoprotein. RGMa is a glycosylphosphatidylinositol-anchored glycoprotein that functions as an axon guidance protein in the developing and adult central nervous system. It helps guide Retinal Ganglion Cell (RGC) axons to the tectum in the midbrain. RGMa has been implicated to play an important role in the developing brain and in the scar tissue that forms after a brain injury. This protein may also function as a tumor suppressor in some cancers.
Reference
  • Severyn CJ, et al. (2009). Molecular biology, genetics and biochemistry of the repulsive guidance molecule family. Biochem J. 422 (3): 393-403.
  • Monnier PP, et al. (2002) RGM is a repulsive guidance molecule for retinal axons. Nature. 419: 392-5.
  • Matsunaga E, et al. (2004) RGM and its receptor neogenin regulate neuronal survival. Nature Cell Biology. 6: 749-55.