Human RHEB Protein (His Tag)

RHEB2

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Catalog Number	P11382-H07B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	RHEB2
Molecular Weight	The recombinant human RHEB consists of 199 amino acids and has a calculated molecular mass of 22.4 kDa. The recombinant protein migrates as an approximately 21 kDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 85 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human RHEB (Q15382)(Met1-Cys181) was fused with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Immunology |Signal Transduction |Metabolism |Types of disease |Metabolism in Obesity
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	RHEB is a recently discovered member of the Ras superfamily that may be involved in neural plasticity. This function is novel and not typically associated with the Ras proteins. RHEB gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. RHEB is vital in regulation of growth and cell cycle progression due to its role in the insulin / TOR / S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of RHEB is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
Reference	Karbowniczek, et al. (2004) Regulation of B-Raf kinase activity by tuberin and Rheb is mammalian target of rapamycin (mTOR)-independent. J Biol Chem. 279(29):29930-7. Long, et al. (2005) Rheb binds and regulates the mTOR kinase. Curr Biol. 15(8):702-13. Mizuki N, et al. (1996) Isolation of cDNA and genomic clones of a human Ras-related GTP-binding protein gene and its chromosomal localization to the long arm of chromosome 7, 7q36. Genomics. 34(1):114-8.