Human RNase A / Ribonuclease A / RNASE1 Protein (His Tag)
RAC1,RIB1,RNS1
- 100ug (NPP2466) Please inquiry
| Catalog Number | P13468-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | RAC1,RIB1,RNS1 |
| Molecular Weight | The recombinant human RNASE1 comprises 139 amino acids and has a predicted molecular mass of 16 kDa. The apparent molecular mass of the protein is approximately 29 ,26 and 22 kDa in SDS-PAGE under reducing conditions |
| predicted N | Lys 29 |
| SDS-PAGE |  |
| Purity | (81.9+12.3+4.7) % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human RNASE1 (P07998) (Met1-Thr156) was expressed with a polyhistidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | Epigenetics |DNA / RNA |RNA Processing |Other in RNA processing |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As a endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. |
| Reference |