Human SAE1 Protein (His & GST Tag)
AOS1,HSPC140,SUA1,UBLE1A
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Catalog Number | P13921-H20B |
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Organism Species | Human |
Host | Baculovirus-Insect Cells |
Synonyms | AOS1,HSPC140,SUA1,UBLE1A |
Molecular Weight | The recombinant human SAE1 /GST chimera consists of 583 amino acids and has a calculated molecular mass of 66.3 kDa. The recombinant protein migrates as an approximately 61 kDa band in SDS-PAGE under reducing conditions. |
predicted N | Met |
SDS-PAGE | |
Purity | > 95 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the human SAE1 (Q9UBE0) (Met1-Lys346) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Bio-activity | |
Research Area | Immunology |Signal Transduction |ITIM/ITAM Immunoreceptors and Related Molecules |
Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 also regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. |
Reference |