Human SELP / selectin P / P-selectin Protein (Fc Tag)
CD62,CD62P,GMP140,GRMP,LECAM3,P-Selectin,PADGEM,PSEL
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| Catalog Number | P13025-H02H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD62,CD62P,GMP140,GRMP,LECAM3,P-Selectin,PADGEM,PSEL |
| Molecular Weight | The recombinant human SELP/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 971 amino acids and has a predicted molecular mass of 107 kDa. The apparent molecular mass of the reduced monomer is approximately 130-150 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Trp 42 |
| SDS-PAGE |  |
| Purity | > 85 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human SELP (AAN06828.1) extracellular domain (Met 1-Ala 771) was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to SELP/Fc Chimera coated plates (10 μg/mL with 100 μL/well), > 80% cells will adhere after 1 hour at 37℃. |
| Research Area | Immunology |Signal Transduction |Cytoskeleton / ECM |Cell Adhesion |Lectin |C-tyep lectin | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | P selectin (SELP) is a 140kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. SELP mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. P selectin is a cell adhesion molecule on the surface of activated endothelial cells. Cellular adhesion molecules are a large family of proteins that attach the cytoskeleton and intracellular signaling cascades with the extracellular environment. SELP is a calcium-dependent receptor for myeloid cells that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interacton of activated endothelial cells or platelets with leukocytes. |
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