Human SH2D1A Protein (His Tag)
DSHP,EBVS,IMD5,LYP,MTCP1,SAP,SAP/SH2D1A,XLP,XLPD,XLPD1
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| Catalog Number | P11149-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | DSHP,EBVS,IMD5,LYP,MTCP1,SAP,SAP/SH2D1A,XLP,XLPD,XLPD1 |
| Molecular Weight | The recombinant human SH2D1A consisting of 139 amino acids and has a calculated molecular mass of 15.6 kDa.It migrates as an approximately 14 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 94 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human SH2D1A isoform 1 (NP_002342.1) (Pro 2-Lys 97) was expressed, with a polyhistidine tag at the N-terminus. |
| Bio-activity | 1. Measured by its ability to bind recombinant human SLAMF1 in a functional ELISA. 2. Measured by its ability to bind recombinant human SLAMF6 in a functional ELISA. 3. Measured by its ability to bind recombinant mouse SLAMF6 in a functional ELISA. 4. Measured by its ability to bind recombinant mouse CD84 in a functional ELISA. |
| Research Area | Signaling |Signal Transduction |Akt Pathway |Adaptor Proteins in the Akt Pathway |
| Formulation | Lyophilized from sterile PBS, pH 7.5, 20% glycerol 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | SH2 domain-containing protein 1A (SH2D1A / SAP) is a 128 amino acid protein, containing a single Src homology 2 (SH2) domain, flanked by 5 amino acids at the N-terminus and 25 amino acids at the C-terminus. The absence of a catalytic domain and the presence of an SH2 domain suggest that SH2D1A regulates one or more signal transduction pathways. SH2D1A interacts with signaling lymphocytic activation molecule (SLAM), which is a transmembrane protein expressed on the surface of activated T and B cells. SH2D1A (SAP) interacts via its SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of the cell-surface receptors, including CD150 / SLAM, CD84, CD229 / Ly-9, and CD244 / 2B4. SH2D1A was expressed in EBV-carrying, tumor phenotype representative (type I), but not in EBV-carrying lymphoblastoid cell line (LCL)-like (type III) or EBV-negative Burkitt lymphoma (BL) lines. It has been supposed to be related to the X-linked lymphoproliferative disease which is also known as Duncan's disease or Purtilo syndrome. |
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