Human SIGIRR / TIR8 Protein (Fc Tag)
SIGIRR
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| Catalog Number | P12165-H02H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | SIGIRR |
| Molecular Weight | The recombinant human SIGIRR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 359 amino acids and has a predicted molecular mass of 39.5 kDa. The apparent molecular mass of the protein is approximately 47-54 and 33 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | (96.8+1.6) % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human SIGIRR (Q6IA17-1) (Met1-His118) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | |
| Research Area | Developmental Biology |Post embryonic development |Cellular Senescence & Aging |Apoptosis |NF-kB (NFkB) Pathway |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Single Ig IL-1-related receptor (SIGIRR) or TIR8 is a member of Toll-like receptor-interleukin 1 receptor signaling (TLR-IL-1R) receptor superfamily. Although SIGIRR/TIR8 shows the typical conserved motifs that characterize the IL-1R and Toll superfamily, it is structurally and functionally distinct from both. SIGIRR/TIR8 has only one Ig domain in its extracellular portion whereas the IL-1R family contains three Ig folds. An unusually long cytoplasmic domain is reminiscent of the structure of drosophila Toll, yet the SIGIRR peptide sequence is more closely related to IL-1RI. SIGIRR/TIR8 was mainly expressed in mouse and human epithelial tissues such as kidney, lung and gut. Resting and activated T and B lymphocytes and monocytes-macrophages expressed little or no SIGIRR/TIR8, with the exception of the mouse GG2EE macrophage line. Inflammation is enhanced in SIGIRR-deficient mice. SIGIRR negatively modulates immune responses. Inflammation is enhanced in SIGIRR-deficient mice, as shown by their enhanced chemokine induction after IL-1 injection and reduced threshold for lethal endotoxin challenge. |
| Reference |