Human SIRPG / SIRP gamma / CD172g Protein (His Tag)
bA77C3.1,CD172g,SIRP-B2,SIRPB2,SIRPgamma
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| Catalog Number | P11828-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | bA77C3.1,CD172g,SIRP-B2,SIRPB2,SIRPgamma |
| Molecular Weight | The recombinant human SIRPG consists of 312 amino acids and predictes a molecular mass of 34.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSIRPG is approximately 45-48 kDa due to glycosylation. |
| predicted N | Val 64 |
| SDS-PAGE |  |
| Purity | > 96 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human SIRPG (NP_061026.2) extracellular domain (Met 1-Ser 364) was expressed, with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc (P12283-H02H), The EC50 of human CD47-Fc (P12283-H02H) is 0.58-1.34 μg/ml. |
| Research Area | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Signal-regulatory protein gamma (SIRPG/SIRP gamma) also known as CD172 antigen-like family member B, CD172g, and CD172g antigen, is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPG/SIRP gamma/CD172g is probable immunoglobulin-like cell surface receptor. On binding with CD47, SIRPG can mediate cell-cell adhesion. SIRPG/SIRP gamma is engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. SIRPG/SIRP gamma/CD172g is detected in liver, and at very low levels in brain, heart, lung, pancreas, kidney, placenta and skeletal muscle. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. This cytokine is mainly present in the paracortical T-cell area of lymph nodes, with only sparse positive cells in the mantle and in the germinal center of B-cell follicles. In the thymus, SIRPG is primarily expressed in the medulla on mature T-lymphocytes that have undergone thymic selection. |
| Reference |