Human SMAC / Diablo Protein (His Tag)
DFNA64,SMAC
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| Catalog Number | P10339-H08E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | DFNA64,SMAC |
| Molecular Weight | The recombinant human Diablo/SMAC consists of 191 amino acids and migrates as polypeptide of 22 kDa as estimated by SDS-PAGE under reduced and non-reduced conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mitochondrial-located form of human Diablo (NP_063940.1) (Ala 56-Asp 239) was expressed with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized recombinant human SMAC-His (P10339-H08E）at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI (P10606-H17E) with a linear range of 0.125-1.0 μg/ml. |
| Research Area | Immunology |Signal Transduction |Other Related Intracellular Topics |Inhibitors of Apoptosis (IAPs) and Regulators |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer. |
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