Human SMYD3 / ZMYND1 Protein (His & FLAG Tag)
bA74P14.1,KMT3E,ZMYND1,ZNFN3A1
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| Catalog Number | P11230-H18H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | bA74P14.1,KMT3E,ZMYND1,ZNFN3A1 |
| Molecular Weight | The recombinant human SMYD3 consists of 447 amino acids and predicts a molecular mass of 51.5 kDa. It migrates as an approxiamtely 49 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human SMYD3 isoform 1 (NP_001161212.1) (Met 1-Ser 428) was fused with the flag tag at the N-terminus. and the Histidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | Cancer |Oncoprotein & suppressor & biomarker |Oncoprotein |Transcription factor |
| Formulation | Lyophilized from sterile 25mM Tris-HCl, 100mM NaCl, 20% glycerol, 3mM DTT, pH 8.0 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains one MYND-type zinc finger and one SET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis. It activates the transcription of a set of downstream genes. Of these downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, migration and metastasis. Increased SMYD3 expression is essential for the proliferation of breast cancer cells. SMYD3 may be a promising new target of therapeutic intervention for the treatment of cancers or other pathological processes associated with cell adhesion and migration. |
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