Human SOCS3 / CIS3 Protein (His & Trx Tag)
ATOD4,CIS3,Cish3,SOCS-3,SSI-3,SSI3
- 100ug (NPP4284) Please inquiry
| Catalog Number | P11315-H30E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | ATOD4,CIS3,Cish3,SOCS-3,SSI-3,SSI3 |
| Molecular Weight | The recombinant human SOCS3/Trx fusion protein consisting of 384 amino acids and has a calculated molecular mass of 41.9 kDa. It migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human SOCS3 (O14543) (Met 1-Leu 225) was fused with a Trx and a polyhistide tag at the N-terminus. |
| Bio-activity | |
| Research Area | Immunology |Signal Transduction |Jak/STAT Signaling |Negative Regulators of the Jak/STAT Pathway |
| Formulation | Lyophilized from sterile 50mM Tris, pH 8.0 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Suppressor of cytokine signaling 3, also known as SOCS-3, Cytokine-inducible SH2 protein 3, CIS-3, STAT-induced STAT inhibitor 3, SOCS3 and CIS3, is a protein which is widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. SOCS3 / CIS3 contains one SH2 domain and one SOCS box domain. SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 / CIS3 is involved in negative regulation of cytokines that signal through the JAK / STAT pathway. SOCS3 / CIS3 inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. SOCS3 / CIS3 suppresses fetal liver erythropoiesis. It regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. SOCS3 / CIS3 regulates IL-6 signaling. SOCS3 / CIS3 interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. SOCS3 / CIS3 could be used as a possible therapeutic agent for treating rheumatoid arthritis. |
| Reference |