Human SPHK1 / Sphingosine Kinase 1 Protein
SPHK,SPHK1,SPK
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| Catalog Number | P15679-HNCB |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | SPHK,SPHK1,SPK |
| Molecular Weight | The recombinant human SPHK1 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The recombinant protein migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Gly |
| SDS-PAGE |  |
| Purity | > 85 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was fused with two additional amino acids (Gly & Pro) at the N-terminus. |
| Bio-activity | The specific activity was determined to be 1028 nmol/min/mg using Sphingosine Kinase Substrate as substrate. |
| Research Area | Cancer |Signal transduction |Metabolism |Types of disease |Metabolism in Cancer |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. |
| Reference |