Human SRC Kinase / Proto-oncogene c-Src Protein (His & GST Tag)
ASV,c-SRC,p60-Src,SRC1
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| Catalog Number | P10755-H20B |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | ASV,c-SRC,p60-Src,SRC1 |
| Molecular Weight | The recombinant human SRC/GST chimera consists of 773 amino acids and has a calculated molecular mass of 87.7 KDa. It migrates as an approximately 81 KDa band in SDS-PAGE under non-reduced conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human SRC (P12931-1) (Met 1-Leu 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Bio-activity | The specific activity was determined to be 27 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. |
| Research Area | Cardiovascular |Angiogenesis |Cytokines / Chemokines in Angiogenesis |TNF Superfamily |TNF Superfamily Regulators |
| Formulation | Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 0.3mM DTT 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity. |
| Reference |