Human SRFBP1 Protein (His & GST Tag)
BUD22,P49,p49/STRAP,Rlb1,STRAP
- 100ug (NPP2512) Please inquiry
| Catalog Number | P14461-H20B |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | BUD22,P49,p49/STRAP,Rlb1,STRAP |
| Molecular Weight | The secreted recombinant human SRFBP1 consists of 666 amino acids and predicts a molecular mass of 76.5 KDa. The apparent molecular mass of the protein is approximately 69 KDa in SDS-PAGE under reducing conditions due to glycosylation. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human SRFBP1(Q8NEF9) (Met1-Asp429) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Bio-activity | |
| Research Area | |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | SRFBP1 contains 7 WD repeats and belongs to the WD repeat STRAP family. SRFBP1 may play a role in the cellular distribution of the SMN complex. The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. SRFBP1 negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein. SRFBP1 may be involved in regulating transcriptional activation of cardiac genes during the aging process. It also may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells. |
| Reference |