Human SRI / Sorcin Protein (His & GST Tag)

CP-22,CP22,SCN,V19

• 100ug (NPP2513) Please inquiry

Catalog Number	P14547-H20B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	CP-22,CP22,SCN,V19
Molecular Weight	The recombinant human SRI /GST chimera consists of 435 amino acids and has a calculated molecular mass of 49.5 kDa. The recombinant protein migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human SRI (P30626) (Met1-Val198) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity
Research Area	Signaling |Signal Transduction |Cytoskeleton / ECM |Cell Adhesion |Tight Junctions
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Sorcin was originally identified in multidrug-resistant cells. It is a alcium-binding protein. Sorcin modulates excitation-contraction coupling in the heart, contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Sorcin is overexpressed in the multi-drug resistant chinese hamster ovary cell line CHRC5 and a variety of multidrug-resistant tumor cell lines, but overexpression is not a sufficient or necessary condition for the acquisition of the multidrug-resistant phenotype.
Reference	Meyers MB, et al. (1995) Association of sorcin with the cardiac ryanodine receptor. J Biol Chem. 270(44):26411-8. Brownawell AM, et al. (1997) Calcium-dependent binding of sorcin to the N-terminal domain of synexin (annexin VII). J Biol Chem. 272(35):22182-90. Hansen, et al. (2003) The PEF family proteins sorcin and grancalcin interact in vivo and in vitro. FEBS Lett. 545(2-3):151-4.