Human STK16 / PKL12 / MPSK Protein (His & NusA Tag)
KRCT,MPSK,PKL12,TSF1
- 100ug (NPP4293) Please inquiry
| Catalog Number | P12201-H24E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | KRCT,MPSK,PKL12,TSF1 |
| Molecular Weight | The recombinant human STK16/NusA chimera consisting of 820 amino acids and has a calculated molecular mass of 92 kDa. It migrates as an 105 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 85 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the native human STK16 (AAH02618.1) (Met 1-Ile 305) was fused with the polyhistidine-tagged NusA tag at the N-terminus. |
| Bio-activity | Kinase activity untested |
| Research Area | Signaling |Signal Transduction |Protein Kinase |Intracellular Kinase |Other Intracellular Protein Kinases |
| Formulation | Supplied as sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Serine/threonine-protein kinase 16, also known as myristoylated and palmitoylated serine/threonine-protein kinase, Protein kinase PKL12, TGF-beta-stimulated factor 1, TSF-1, MPSK1 and STK16, is a membrane protein which is ubiquitously expressed at very low levels. STK16 / MPSK1 belongs to the protein kinase superfamily and Ser/Thr protein kinase family. It contains one protein kinase domain. Transforming growth factor-beta (TGF-beta) shows a variety of biological activities in various organs or cells. Some factors such as Smads (Sma and Mad proteins) and TGF-beta activating kinase 1 have been characterized as signalling molecules downstream of TGF-beta. Several TGF-beta response elements have been identified such as cAMP response element, Smad binding element, and recognition sites for activating protein-1 and stimulating protein-1 in various gene promoters. STK16 / MPSK1 is an unique factor with two biological functions, transcriptional regulation and protein phosphorylation, that may be involved in TGF-beta signals. STK16 / MPSK1 is a protein kinase that act on both serine and threonine residues. STK16 / MPSK1 possessed DNA-binding ability and activated the TGF-beta responsive CNP promoter or vascular endothelial growth factor gene promoter which possesses a sequence element analogous to the TGF-beta responsive GC-rich element of the CNP promoter. STK16 / MPSK1 did not directly activate a Smads-dependent promoter from plasminogen activator inhibitor 1 gene, but it showed enhancement in co-operation with Smad3 and Smad4. STK16 / MPSK1 mRNA as well as its protein level were stimulated by TGF-beta treatment. |
| Reference |