Human STK24 / MST3 Protein (His Tag)

HEL-S-95,MST3,MST3B,STE20,STK3

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Catalog Number	P13188-H07B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	HEL-S-95,MST3,MST3B,STE20,STK3
Molecular Weight	The recombinant human STK24 consists of 450 amino acids and predicts a molecular mass of 50.3 kDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 82 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human STK24 isoform A (Q9Y6E0-2) (Met 1-His 431) was expressed, with a polyhistidine tag at the N-terminus.
Bio-activity	The specific activity was determined to be 62 nmol/min/mg using synthetic PKCtide peptide (ERMRPRKRQGSVRRRV) as substrate.
Research Area	Signaling |Signal Transduction |Protein Kinase |Intracellular Kinase |Other Intracellular Protein Kinases
Formulation	Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 0.1mM EGTA, 0.1mM EDTA, 0.25mM DTT 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Lu TJ, et al. (2006) Inhibition of cell migration by autophosphorylated mammalian sterile 20-like kinase 3 (MST3) involves paxillin and protein-tyrosine phosphatase-PEST. J Biol Chem. 281(50): 38405-17. Lu TJ, et al. (2005) Zinc ion acts as a cofactor for serine/threonine kinase MST3 and has a distinct role in autophosphorylation of MST3. J Inorg Biochem. 99(6): 1306-13. Schinkmann K, et al. (1997) Cloning and characterization of a human STE20-like protein kinase with unusual cofactor requirements. J Biol Chem. 272 (45): 28695-703.