Human SUB1 Protein (His Tag)
p14,P15,PC4,RPO2TC1,SUB1
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| Catalog Number | P14326-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | p14,P15,PC4,RPO2TC1,SUB1 |
| Molecular Weight | The recombinant human SUB1 consists of 142 amino acids and predicts a molecular mass of 16.2 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | His |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mature form of human SUB1 (P53999) (Met1-Leu127) was expressed with a polyhistide tag at the N-terminus. |
| Bio-activity | |
| Research Area | Epigenetics |Transcription |Polymerase associated factors |Pol II Transcription |Other in Pol II Transcription |
| Formulation | Lyophilized from sterile PBS, 10% glycerol, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | SUB1 belongs to the transcriptional coactivator PC4 family. It is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 binds single-stranded DNA. Single-stranded DNA-binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. SUB1 exhibits strong genetic interactions with factors necessary for promoter melting. It localizes near the transcription bubble in vitro and binds to promoters in vivo dependent upon preinitiation complexes assembly. SUB1 interacts with the nontemplate strand of RNApII complexes during initiation. It may also be involved in stabilizing the multiprotein transcription complex. |
| Reference |