Call Now

Human Semaphorin 4A / SEMA4A / Semaphorin B Protein (Fc Tag)

CORD10,RP35,SEMAB,SEMB

Catalog Number P11756-H02H
Organism Species Human
Host Human Cells
Synonyms CORD10,RP35,SEMAB,SEMB
Molecular Weight The secreted recombinant human SEMA4A/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 893 amino acids and predictes a molecular mass of 99 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA4A/Fc monomer is approximately 110 kDa due to glycosylation.
predicted N Gly 32
SDS-PAGE
Purity > 90 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human SEMA4A (NP_071762.2) extracellular domain (Met 1-His 683) was fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity Measured by its ability to bind mouse SEMA4D in a functional ELISA.
Research Area Cancer |Invasion microenvironment |Angiogenesis |Angiogenesis Inhibitor
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference
  • Clark H.F., et al., 2003, Genome Res. 13: 2265-2270.
  • Ota T., et al., 2004,Nat. Genet. 36: 40-45.
  • Neufeld, G. et al., 2005, Front Biosci. 10 : 751-60.
  • Fiore,R. et al., 2005, Mol Cell Biol. 25 (6):2310-9.
  • Abid A., et al., 2006, J. Med. Genet. 43:378-381.