Human Semaphorin 4A / SEMA4A / Semaphorin B Protein (His Tag)

CORD10,RP35,SEMAB,SEMB

Catalog Number	P11756-H08H
Organism Species	Human
Host	Human Cells
Synonyms	CORD10,RP35,SEMAB,SEMB
Molecular Weight	The recombinant human SEMA4A consists of 663 amino acids and predictes a molecular mass of 73.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA4A is approximately 75-80 kDa.
predicted N	Gly 32
SDS-PAGE
Purity	> 96 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human SEMA4A (NP_071762.2) extracellular domain (Met 1-His 683) was expressed, with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to bind biotinylated mouse SEMA4D in a functional ELISA.
Research Area	Cancer \|Invasion microenvironment \|Angiogenesis \|Angiogenesis Inhibitor
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Clark H.F., et al., 2003, Genome Res. 13: 2265-2270. Ota T., et al., 2004,Nat. Genet. 36: 40-45. Neufeld, G. et al., 2005, Front Biosci. 10 : 751-60. Fiore,R. et al., 2005, Mol Cell Biol. 25 (6):2310-9. Abid A., et al., 2006, J. Med. Genet. 43:378-381.