Human SerpinB2 / PAI-2 Protein (GST Tag)
HsT1201,PAI,PAI-2,PAI2,PLANH2
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| Catalog Number | P10641-H09B |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | HsT1201,PAI,PAI-2,PAI2,PLANH2 |
| Molecular Weight | The recombinant human SERPINB2/GST chimera consists of 640 amino acids and predicts a molecular mass of 73 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the full length of human SERPINB2 (NP_002566.1) (Met 1-Pro 415) was fused with the GST tag at the N-terminus. |
| Bio-activity | Immobilized human GST-SerpinB2 at 10 μg/ml (100 μl/well) can bind biotinylated human uPA-His (P10815-H08H), The EC50 of biotinylated human uPA-His (P10815-H08H) is 10.24-23.88 ng/ml. |
| Research Area | Immunology |Innate Immunity |Coagulation |
| Formulation | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM Reduced Glutathione, 10% glycerol, 0.5mM PMSF 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Serpins are the largest and most diverse family of serine protease inhibitors which are involved in a number of fundamental biological processes such as blood coagulation, complement activation, fibrinolysis, angiogenesis, inflammation and tumor suppression and are expressed in a cell-specific manner. SerpinB2, also known as Plasminogen activator inhibitor 2, Placental plasminogen activator inhibitor, Monocyte Arg-serpin, Urokinase inhibitor and PAI2, is a cytoplasm protein which belongs to the serpin family and Ov-serpin subfamily. SerpinB2 is a major product of activated monocytes and macrophages and is substantially induced during most inflammatory processes. Distinct from its widely described extracellular role as an inhibitor of urokinase plasminogen activator. SerpinB2 has been shown to have an intracellular role as a retinoblastoma protein (Rb)-binding protein that inhibits Rb degradation. SerpinB2 is widely described as an inhibitor of urokinase plasminogen activator. SerpinB2 inhibits urokinase-type plasminogen activator. The monocyte derived SerpinB2 is distinct from the endothelial cell-derived PAI-1. SerpinB2 is a potentially important inducible host factor that significantly promotes HIV-1 replication. |
| Reference |