Human TBCB Protein (His Tag)

CG22,CKAP1,CKAPI

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Catalog Number	P14260-H07E
Organism Species	Human
Host	E. coli
Synonyms	CG22,CKAP1,CKAPI
Molecular Weight	The recombinant human TBCB consists of 259 amino acids and predicts a molecular mass of 29.2 KDa. It migrates as an approximately 33-37 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human TBCB (Q99426) (Met1-Ile244) was expressed with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Immunology |Signal Transduction |Cytoskeleton / ECM |Cytoskeletal Proteins |Microtubules |Tubulin |
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Tubulin-folding cofactor B, also known as TBCB, belongs to the TBCB family. It contains 1 CAP-Gly domain and can be detected in most tissues. TBCB binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway. The cytoskeleton is composed of 3 structural elements: actin filaments, microtubules, and intermediate filaments. TBCB is involved in regulation of tubulin heterodimer dissociation. It may function as a negative regulator of axonal growth.
Reference	Feingold EA, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903. Tian G, et al. (1997) Tubulin subunits exist in an activated conformational state generated and maintained by protein cofactors. J Cell Biol. 138(4):821-32. Wolz W, et al. (1997) A complex satellite DNA polymorphism flanking the human ryanodine receptor gene (RYR1). Cytogenet Cell Genet. 72(2-3):215-6.