Human TCN2 Protein (His Tag)
D22S676,D22S750,II,TC,TC-2,TC2,TCII
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| Catalog Number | P10566-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | D22S676,D22S750,II,TC,TC-2,TC2,TCII |
| Molecular Weight | The secreted recombinant human TCN2 comprises 418 amino acids with a predicted molecular mass of 46.7 kDa. It migrates with the molecular weight of approximately 43 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Glu 19 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human TCN2 (NP_000346.2) (Met 1-Trp 427) with a C-terminal polyhistidine tag was expressed. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human TCN2-His at 10μg/mL (100μL/well) can bind biotinylated mouse CD320-His (P51138-M08H). The EC50 of biotinylated mouse CD320-His(P51138-M08H) is 18-42 ng/mL. |
| Research Area | Cancer |Signal transduction |Metabolism |Pathways and Processes |Vitamins / minerals |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities. |
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