Human TGFA / TGF-alpha Protein
TFGA,TGFa
- 100ug (NPP1690) Please inquiry
| Catalog Number | P11252-HNAE |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | TFGA,TGFa |
| Molecular Weight | The recombinant human TGFa consists of 50 amino acids and has a predicted molecular mass of 5.6 kDa. The apparent molecular mass of it is approximately 13 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human TGFa (P01135-1) (Val40-Ala89) was expressed and purified. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. Immobilized human TGFa (cat:11252-HNAE) at 10 μg/ml (100 μl/well) can bind human EGFR-Fc (P10001-H02H). The EC50 of human EGFR-Fc (P10001-H02H) is 20-60 ng/ml. 2. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.1-0.5ng/mL. |
| Research Area | Immunology |Cytokines & Growth Factors |Growth Factor & Receptor |Epidermal Growth Factor (EGF) & Receptor |Epidermal Growth Factor (EGF) |
| Formulation | Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |