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Human TIGIT / VSTM3 Protein (His Tag)

VSIG9,VSTM3,WUCAM

Catalog Number P10917-H08H
Organism Species Human
Host Human Cells
Synonyms VSIG9,VSTM3,WUCAM
Molecular Weight The secreted recombinant human TIGIT comprises 128 amino acids with a predicted molecular mass of 14.2 kDa.
predicted N Met 22
SDS-PAGE
Purity > 85 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the extracellular domain of human TIGIT (NP_776160.2) (Met 1-Phe 138) with a C-terminal polyhistidine tag was expressed.
Bio-activity 1. Measured by its ability to bind with human CD155-Fc (P10109-H02H) in a functional ELISA.
2. Measured by its ability to bind with mouse PVR-Fch (P50259-M03H) in a functional ELISA.
Research Area Immunology |Adaptive Immunity |T Cell |Non-CD of T cell
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.
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