Human TLE3 Protein (aa 484-772, GST Tag)

ESG,ESG3,GRG3,HsT18976

• 100ug (NPP2540) Please inquiry

Catalog Number	P13099-H09E
Organism Species	Human
Host	E. coli
Synonyms	ESG,ESG3,GRG3,HsT18976
Molecular Weight	The recombinant human TLE3/GST chimera consists of 520 amino acids and has a predicted molecular mass of 58.5 kDa as estimated in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 78 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the N-terminal fragment of human TLE3 (Q04726-1) (Ser 484-Tyr 772) was fused with the GST tag at the N-terminus.
Bio-activity
Research Area	Signaling |Signal Transduction |Signaling Pathway |Nuclear signaling |Nuclear Hormone Receptor |Co-activator & Co-repressor |
Formulation	Lyophilized from sterile 20mM Tris, 0.15M NaCl, 5mM GSH, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Villanueva CJ, et al. (2011) TLE3 is a dual-function transcriptional coregulator of adipogenesis. Cell Metab. 13(4): 413-27. Carvalho LR, et al. (2010) Corepressors TLE1 and TLE3 interact with HESX1 and PROP1. Mol Endocrinol. 24(4): 754-65. Nakaya HI, et al. (2007) Splice variants of TLE family genes and up-regulation of a TLE3 isoform in prostate tumors. Biochem Biophys Res Commun. 364(4): 918-23. Brinkmeier ML, et al. (2003) TCF and Groucho-related genes influence pituitary growth and development. Mol Endocrinol. 17: 2152-2161. Dasen JS, et al. (2001) Temporal regulation of a paired-like homeodomain repressor/TLE corepressor complex and a related activator is required for pituitary organogenesis. Genes Dev. 15: 3193-3207. Douglas KR, et al. (2001) Identification of members of the Wnt signaling pathway in the embryonic pituitary gland. Mamm Genome. 12: 843-851. Chen G, et al. (2000) Groucho/TLE family proteins and transcriptional repression. Gene. 249: 1-16.