Human TLK1 / PKU-beta Protein (His & GST Tag)

KIAA0137, PKU-beta

Catalog Number	P12213-H20B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	KIAA0137, PKU-beta
Molecular Weight	The recombinant human TLK1/GST chimera consists of 1003 amino acids and has a calculated molecular mass of 114 kDa. It migrates as an approximately 125 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 88 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human TLK1 isoform 1 (Q9UKI8-1) (Met 1-Tyr 766) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity	The specific activity was determined to be 1.6 nmol/min/mg using Histone H3 protein as substrate.
Research Area	Cancer \|Signal transduction \|Protein Kinase \|Intracellular Kinase \|Other Intracellular Protein Kinases
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Sillje H.H.W., et al., 2001, Curr. Biol. 11:1068-73. Beausoleil S.A., et al., 2004, Proc. Natl. Acad. Sci. USA. 101:12130-5. Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64. Mayya V., et al., 2009, Sci. Signal. 2: RA46-RA46.