Human TMEM27 Protein (His Tag)

NX-17,NX17

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Catalog Number	P13301-H08H
Organism Species	Human
Host	Human Cells
Synonyms	NX-17,NX17
Molecular Weight	The recombinant human TMEM27 consists of 138 amino acids and predicts a molecular mass of 15.8 KDa. It migrates as an approximately 27 and 33 KDa band in SDS-PAGE under reducing conditions.
predicted N	Glu 15
SDS-PAGE
Purity	(48.3+41.6) % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human TMEM27 (NP_862830.1) (Met1-Pro141) was expressed with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area	Neuroscience |Neurotransmission |Intracellular Signaling |Regulation
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	TMEM27 is a membrane protein. It has been proposed as a beta cell mass biomarker since it is cleaved and shed by pancreatic beta cells. Overexpression of TMEM27 leads to increased thymidine incorporation, whereas silencing of Tmem27 using RNAi results in a reduction of cell replication. Furthermore, transgenic mice with increased expression of Tmem27 in pancreatic beta cells exhibit increased beta cell mass. TMEM27 is also important for trafficking amino acid transporters to the apical brush border of proximal tubules.
Reference	Pasquali L, et al. (2009) Collectrin gene screening in Turner syndrome patients with kidney malformation. J Genet. 88(1):105-8. Tosetto E, et al. (2009) Locus heterogeneity of Dent's disease: OCRL1 and TMEM27 genes in patients with no CLCN5 mutations. Pediatr Nephrol. 24(10):1967-73. Singer D, et al. (2011) Collectrin and ACE2 in renal and intestinal amino acid transport. Channels (Austin). 5(5):410-23.