Human TROP2 / TACSTD2 Protein (His & Fc Tag)
EGP-1,EGP1,GA733-1,GA7331,GP50,M1S1,TACD2,TROP-2,TROP2
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| Catalog Number | P10428-H03H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | EGP-1,EGP1,GA733-1,GA7331,GP50,M1S1,TACD2,TROP-2,TROP2 |
| Molecular Weight | The recombinant human TROP2/Fc is a disulfide-linked homodimer. The reduced monomer consists of 495 amino acids and has a predicted molecular mass of 55.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTROP2/Fc monomer is approximately 65-75 kDa due to glycosylation. |
| predicted N | His 27 |
| SDS-PAGE |  |
| Purity | > 98 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Thr 274) of human TROP2 (NP_002344.2) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells . When cells are added to TROP2-coated plates (10 μg/ml, 100 μl/well), approximately 90%-100% will adhere specifically . |
| Research Area | Cell Biology |Cell Cycle |Cell division |Other cell division related protein |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | TROP-2, also referred to as tumor associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. |
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